Why autoclave glucose separately

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This is a preview of subscription content, access via your institution. Rent this article via DeepDyve. Chardonnay et division des cellules. OIV — — Google Scholar. Burger DW Guidelines for autoclaving liquid media used in plant tissue culture.

HortScience — Article Google Scholar. Plant Sci. Food Chem. Plant Cell Rep. Moye CJ 5-hydroxymethylfurfural. Pure Appl. If the solution contains compounds that react or change with high temperature like that of autoclave sterilizer, it is reasonable not to use it.

A good example is the cell culture medium which contains vitamins, amino acids and salt. The only compounds that may escape destruction or denaturation are the salt solution, therefore you cant autoclave-sterilize your cell culture medium. You just need to filter it. This idea can be applied to any other solution you might want to sterilize in the future. You must be able to answer the question " How will this solution react to autoclave sterilizer temperature?

Whenever I want to sterilize, I make sure I give that question a befitting answer and I go ahead with the best option. You can do the same. I autoclave my solutions separately and there is no color change. Initially,i used to filter sterile my sugar solution with 0.

As mentioned,after autoclaving, i added my phosphate and sugar solutions after cooling and my microorganisms just love it. Glucose is a reducing sugar and can react with amino acids in high temperatures Maillard reaction. If you don't have sterile filters I would recommend to autoclave a glucose solution separately and mix it afterwards.

In our aerobic wastewater treatment tank, there is growth of pink worms as shown in the picture. Any idea what those might be?

Would it affect our reactor? How to remove and avoid this We are designing a comparative analysis between an infected group and a healthy group but we are unsure as to how much patients we would need to have a meaningful comparison. If I have an object which results I would like to be created as a file, how can I do it?

It is my first time handling plasma samples. For a standard, we need to use a human plasma that have been lyophilized. What is the proper dissolution for it and what is the solvent? How much of We received a plasmid delivered by mail and performed transformation experiment following protocol. Would it make any trouble if I we use phosphate buffer instead of TE buffer in diluting Nucleic acids?

If I am correct, phosphate buffer is used typically to maintain the viability of live cells We are planning to resuspend a fungal culture with phosphate buffer and add an energy source to sustain life while removing other compounds in a medium that might interfere with the possible I've been looking at possible effects of pharmaceuticals on nitrifiers.

Though it shows a difference in the different nitrogenous compounds, our observations didn't show any correlation with the Can someone please give me some possible things that could go wrong? Here is my recipe: 0. Gel was run at V for 1 hour. The buffer used is also TAE. Many others in the lab do it like this as well. I Always put some water lets say ml in my bottle, with a magnet, and then I add the glucose, gram per time. Think I recall glucose may a bit less stable with heat and doubt if there's much data for it's stability in routine media but enough apparently remains to be functional.

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